CAR-T Development Lab

Design receptors · challenge tumors · compare chassis

Educational cell-therapy workbench

Build a CAR. Stress-test it on model tumors. Rank T-cell phenotypes.

Tune antigens, hinges, transmembrane anchors, and costim modules — save protocols and present a full-screen demo deck.

01
Design
Modules & dose
02
Engage
Cartoon synapse
03
Present
Demo mode
Live construct
AgCD19Costim4-1BBTMCD8αDose10^6.5
Match
90%
Surface
94%
Best
Tscm
Quick start protocols
Saved protocols
No saved protocols yet. Design a construct, then for demos and reloads (stored in this browser).
Simulation canvas
Live
Antigen match
90%
Surface CAR (TM)
94%
Best chassis
Tscm
Off-tumor risk
35%

Engagement stage

Cartoon synapse, kill pressure, and transmembrane readouts for the selected chassis.

Interactive
Active killing synapse

CAR scFv bound to tumor antigen; perforin/granzyme and cytokine programs active.

CAR-T · Tscm
Stem cell memory (Tscm)
8 surface CARs
Kill pressure
65%
scFv → CD19
Tumor · CD19
B-ALL
Membrane stress / apoptosis
Antigen match
90%
Target fit
Surface CAR
94%
CD8α TM
TM coupling
77%
Across membrane
TM stability
95%
Tonic load OK
Transmembrane effect

Stable clinical default. High surface CAR density, low tonic signaling, solid persistence.

Hinge: CD8α hingeCostim: 4-1BB (CD137)Dose: 10^6.5 cellsTonic load: 27%

CAR cassette (N → C)

Extra → intra
scFv
CD19
Antigen binder
Hinge
CD8α
Spacer length
TM
CD8α
Membrane anchor
Costim
4-1BB
2nd signal
Signal
CD3ζ
CD3ζ ITAMs

TM note: Stable clinical default. High surface CAR density, low tonic signaling, solid persistence.

T-cell subtype ranking

Same CAR across eight starting phenotypes. Click a bar or row to focus the stage.

SubtypeScore
Tscm
100
Tcm
98
Tn
97
γδ
95
Tem
94
NK
93
Teff
89
Treg
54

Stem cell memory (Tscm)

Capability radar

Metric breakdown

Normalized 0–100 educational scores

Cytotoxicity74
Expansion87
Persistence80
Tumor control97
Exhaustion risk10
CRS risk63

Educational simulation of CAR architecture trade-offs. Not for clinical use, protocol design, or patient decisions. Saved protocols stay in this browser only.

CAR-T Lab · v1.1